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Western blot analysis in human placenta tissue lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained wit.U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly.
Western blot analysis in human placenta tissue lysates (35ug/lane).

NCL Antibody

CATALOG NUMBER: 57-876

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human NCL.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
77 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCL
Additional Names:
Nucleolin, Protein C23, NCL
Protein Accession Number:
P19338
Protein Gi Number:
90110781
Ncbi Gene Id Number:
4691
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, isinvolved in the synthesis and maturation of ribosomes. It islocated mainly in dense fibrillar regions of the nucleolus. HumanNCL gene consists of 14 exons with 13 introns and spansapproximately 11kb. The intron 11 of the NCL gene encodes a smallnucleolar RNA, termed U20.
Background Reference 1:
Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010)
Background Reference 2:
Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010)
Background Reference 3:
Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010)
Background Reference 4:
Bertrand, L., et al. J. Virol. 84(1):109-118(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-876

List Size:
400 ul

List Price:

$495.00

Datasheet
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