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Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.
Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.
Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

NCLN Antibody

CATALOG NUMBER: 6789

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN.
The immunogen is located within amino acids 390 - 440 of NCLN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Purification:
NCLN Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NCLN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCLN
Additional Names:
NCLN Antibody: NET59, Nicalin, Nicastrin-like protein
Protein Accession Number:
NP_064555
Protein Gi Number:
51873031
Ncbi Gene Id Number:
56926
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
Background Reference 1:
Haffner C, Frauli M, Topp S, et al. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 2004; 23:3041-50.
Background Reference 2:
Dettmer U, Kuhn PH, Abou-Ajram C, et al. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J. Biol. Chem. 2010; 285:26174-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6789

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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