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<strong>Figure 1: Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2: Western Blot Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3: Western Blot Validation in Rat Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: NCOA4 9663, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4: Immunofluorescence Validation of NCOA4 in Daudi Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with 9663 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5: Immunohistochemistry Validation of NCOA4 in Human lung</strong><br>Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6: Immunohistochemistry Validation of NCOA4 in Mouse lung</strong><br>Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lung</strong><br>Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (9663) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1: Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: NCOA4 9663, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

NCOA4 (IN) Antibody

CATALOG NUMBER: 9663

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: monkey (100%), gorilla (100%), dog (100%), horse (100%)
Immunogen:
Anti-NCOA4 antibody (9663) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
WB: 0.5-1 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1202 – Daudi Cell Lysate
Positive Control 2:
Cat. No. 1402 – Mouse Lung Lysate
Predicted Molecular Weight:
Predicted: 70-74 kD

Observed: 70-74 kD
Isoforms:
Human NCOA4 has 4 isoforms, including isoform alpha (614aa, 69.7kD), isoform beta (287aa, 32.6kD), isoform 3 (630aa, 71.4kD) and isoform 4 (650aa, 73.6kD). Mouse NCOA4 has at least one isoform (625aa, 70.4kD). Rat NCOA4 has one isoform (622aa, 70.3kD). 9663 can detect NCOA4 in human, mouse and rat.
Purification:
NCOA4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NCOA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCOA4
Additional Names:
NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG
Protein Accession Number:
Q13772
Protein Gi Number:
8928226
Ncbi Gene Id Number:
8031
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NCOA4 Antibody: Study showed that NCOA4-mediated ferritin complex degradation supplies iron to mitochondria. Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines. NCOA4-mediated ferritinophagy promoted inflammatory responses in periodontitis. Low expression of ferritinophagy-related NCOA4 gene in relation to unfavorable outcome and defective immune cells infiltration in clear cell renal carcinoma.
Background Reference 1:
Fujimaki et al. Mol Cell Biol. 2019; 39(14): e00010-19
Background Reference 2:
Zhang et al. Oncogene. 2021; 40(8): 1425-1439
Background Reference 3:
Guo et al. J Periodontal Res. 2021; 56(3): 523-534
Background Reference 4:
Mou et al. BMC Cancer. 2021; 21(1):18

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9663

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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