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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human brain lysate Lysates/proteins at 20 ug per lane.

NCOA7 Antibody

CATALOG NUMBER: 63-996

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
106 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCOA7
Additional Names:
Nuclear receptor coactivator 7, 140 kDa estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1, NCOA7, ERAP140, ESNA1
Protein Accession Number:
Q8NI08
Protein Gi Number:
110287684
Ncbi Gene Id Number:
135112
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Background Reference 1:
Arai, H., et al. Oncol. Rep. 19(6):1381-1388(2008)
Background Reference 2:
Liu, Y., et al. Schizophr. Res. 89 (1-3), 360-361 (2007) :
Background Reference 3:
Durand, M., et al. BMC Cell Biol. 8, 13 (2007) :
Background Reference 4:
Mungall, A.J., et al. Nature 425(6960):805-811(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-996

List Size:
400 ul

List Price:

$495.00

Datasheet
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