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Antibody is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Fluorescent confocal image of Hela cell stained with (MOUSE) Ncor1 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Ncor1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Ncor1 immunoreactivity is localized to nucleus significantly.
Antibody is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.

NCOR1 Antibody

CATALOG NUMBER: 55-711

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This NCOR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from the N-terminal region of human NCOR1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
270 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCOR1
Additional Names:
Nuclear receptor corepressor 1, N-CoR, N-CoR1, NCOR1, KIAA1047
Protein Accession Number:
O75376
Ncbi Gene Id Number:
9611
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NCOR1 mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. The gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.
Background Reference 1:
Tai, H.H., et al., Biochem. Biophys. Res. Commun. 308(1):170-176 (2003).
Background Reference 2:
Ishizuka, T., et al., Mol. Cell. Biol. 23(15):5122-5131 (2003).
Background Reference 3:
Loinder, K., et al., J. Steroid Biochem. Mol. Biol. 84(1):15-21 (2003).
Background Reference 4:
Wang, S., et al., Oncogene 21(55):8388-8396 (2002).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-711

List Size:
400 ul

List Price:

$495.00

Datasheet
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