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Western blot analysis of NDFIP1 in PC-3 cell lysate with NDFIP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of NDFIP1 in human lung tissue with NDFIP1 antibody at 5 μg/ml.
Immunofluorescence of NDFIP1 in human lung tissue with NDFIP1 antibody at 20 μg/ml.
Western blot analysis of NDFIP1 in PC-3 cell lysate with NDFIP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

NDFIP1 Antibody

CATALOG NUMBER: 6977

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (84%)
Immunogen:
NDFIP1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NDFIP1.
The immunogen is located within the first 50 amino acids of NDFIP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NDFIP1 antibody can be used for detection of NDFIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NDFIP1 antibody is predicted to not cross-react with NDFIP2.
Positive Control 1:
Cat. No. 1216 - PC-3 Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
24 kDa
Purification:
NDFIP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NDFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NDFIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDFIP1
Additional Names:
NDFIP1 Antibody: N4WBP5, N4WBP5, PSEC0192, PSEC0223, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M
Protein Accession Number:
NP_085048
Protein Gi Number:
13386480
Ncbi Gene Id Number:
80762
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NDFIP1 Antibody: The NEDD4 family-interacting protein 1 (NDFIP1) belongs to a small group of evolutionarily conserved proteins with three transmembrane domains and is an integral Golgi membrane protein. It is a potential target for ubiquitination by the Nedd4 family of proteins. NDFIP1 is strongly expressed in surviving neurons following acute cortical brain injury, and overexpression in cultured cortical neurons increased survival following growth factor starvation, suggesting that NDFIP1 may play a role in neuronal survival. NDFIP1 and the related protein NDFIP2 are thought to interact with and regulate multiple components of the EGF and PTEN/Akt signaling pathways. Recent studies suggest that NDFIP1 may also play a role in Th17 differentiation by limiting the production of proinflammatory cytokines.
Background Reference 1:
Harvey KF, Shearwin-Whyatt LM, Fotia A, et al. N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J. Biol. Chem. 2002; 277:9307-17.
Background Reference 2:
Sang Q, Kim MH, Kumar S, et al. Nedd4-WW domain-binding protein 5 (Ndfip1) is associated with neuronal survival after acute cortical brain injury. J. Neurosci. 2006; 26:7234-44.
Background Reference 3:
Mund T and Pelham HR. Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2. Proc. Natl. Acad. Sci. USA 2010; 107; 11429-34.
Background Reference 4:
Ramon HE, Beal AM, Liu Y, et al. The E3 ubiquitin ligase adaptor Ndfip1 regulates Th17 differentiation by limiting the production of proinflammatory cytokines. J. Immunol. 2012; epub.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6977

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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