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42-420 (0.1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-420 (0.3ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-420 (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-420 (0.1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

NDRG2 Antibody

CATALOG NUMBER: 42-420

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: KEAELAARILLDQGQ-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kDa according to NP_963293.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize reported isoform a (NP_963293.1) only. Reported variants represent identical protein: NP_963831.1, NP_963293.1, NP_963834.1, NP_963833.1.
Positive Control 1:
Cat. No. 1378 - Human Temporal Lobe Tissue Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 3:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NDRG2
Additional Names:
NDRG2, NDRG family member 2, DKFZp781G1938, FLJ25522, KIAA1248, SYLD, N-myc downstream regulator 2, NDR1-related protein NDR2, OTTHUMP00000164405, OTTHUMP00000164406, OTTHUMP00000164407, OTTHUMP00000164408, cytoplasmic protein Ndr1, protein NDRG2, syld709613 protein
Protein Accession Number:
NP_963293.1
Protein Gi Number:
42544211
Ncbi Gene Id Number:
57447

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-420

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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