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Western blot analysis in CEM cell line lysates (35ug/lane).
NDST1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

NDST1 Antibody

CATALOG NUMBER: 56-931

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This NDST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-849 amino acids from the C-terminal region of human NDST1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
101 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDST1
Additional Names:
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, NDST-1, N-heparan sulfate sulfotransferase 1, N-HSST 1, [Heparan sulfate]-glucosamine N-sulfotransferase 1, HSNST 1, Heparan sulfate N-deacetylase 1, 3---, Heparan sulfate N-sulfotransferase 1, 282-, NDST1, HSST, HSST1
Protein Accession Number:
P52848
Ncbi Gene Id Number:
3340
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.
Background Reference 1:
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Background Reference 2:
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Background Reference 3:
Duelli, A., et al. J. Immunol. 183(11):7073-7083(2009)
Background Reference 4:
Zuberi, R.I., et al. J. Immunol. 183(6):3971-3979(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-931

List Size:
400 ul

List Price:

$495.00

Datasheet
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