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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

NDST4 Antibody

CATALOG NUMBER: 59-647

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NDST4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the N-terminal region of human NDST4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
101 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDST4
Additional Names:
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4, Glucosaminyl N-deacetylase/N-sulfotransferase 4, NDST-4, N-heparan sulfate sulfotransferase 4, N-HSST 4, Heparan sulfate N-deacetylase 4, 3---, Heparan sulfate N-sulfotransferase 4, 282-, NDST4, HSST4
Protein Accession Number:
Q9H3R1
Protein Gi Number:
74718249
Ncbi Gene Id Number:
64579
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity (By similarity).
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Background Reference 3:
Treutlein, J., et al. Arch. Gen. Psychiatry 66(7):773-784(2009)
Background Reference 4:
Aikawa, J., et al. J. Biol. Chem. 276(8):5876-5882(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-647

List Size:
400 ul

List Price:

$495.00

Datasheet
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