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Western blot analysis in A2058 cell line lysates (35ug/lane).
Western blot analysis in A2058 cell line lysates (35ug/lane).

NDUFA12 Antibody

CATALOG NUMBER: 60-092

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NDUFA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human NDUFA12.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDUFA12
Additional Names:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13 kDa differentiation-associated protein, Complex I-B172, CI-B172, CIB172, NADH-ubiquinone oxidoreductase subunit B172, NDUFA12, DAP13
Protein Accession Number:
Q9UI09
Protein Gi Number:
12229870
Ncbi Gene Id Number:
55967
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Background Reference 1:
Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)
Background Reference 2:
Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)
Background Reference 3:
Murray, J., et al. J. Biol. Chem. 278(16):13619-13622(2003)
Background Reference 4:
Hu, R.M., et al. Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-092

List Size:
400 ul

List Price:

$495.00

Datasheet
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