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Western blot analysis of extracts of various cell lines, using NDUFA2 antibody (23-424) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human esophagus using NDUFA2 antibody (23-424) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using NDUFA2 antibody (23-424) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using NDUFA2 antibody (23-424) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

NDUFA2 Antibody

CATALOG NUMBER: 23-424

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human NDUFA2 (NP_002479.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
Mouse kidney
Positive Control 2:
Mouse heart
Predicted Molecular Weight:
Observed: 11kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NDUFA2
Additional Names:
B8, CD14, CIB8, MC1DN13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa, NADH-ubiquinone oxidoreductase subunit CI-B8, complex I B8 subunit
Ncbi Gene Id Number:
4695
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-424

List Size:
100 uL

List Price:

$436.00

Datasheet
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