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Western blot analysis in HL-60 cell line lysates (35ug/lane).
NDUFAF4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

NDUFAF4 Antibody

CATALOG NUMBER: 56-779

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NDUFAF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human NDUFAF4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDUFAF4
Additional Names:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, Hormone-regulated proliferation-associated protein of 20 kDa, NDUFAF4, C6orf66, HRPAP20
Protein Accession Number:
Q9P032
Protein Gi Number:
30912745
Ncbi Gene Id Number:
29078
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NADH:ubiquinone oxidoreductase (complex I) catalyzes thetransfer of electrons from NADH to ubiquinone (coenzyme Q) in thefirst step of the mitochondrial respiratory chain, resulting in thetranslocation of protons across the inner mitochondrial membrane.This gene encodes a complex I assembly factor. Mutations in thisgene are a cause of mitochondrial complex I deficiency. [providedby RefSeq].
Background Reference 1:
Saada, A., et al. Am. J. Hum. Genet. 84(6):718-727(2009)
Background Reference 2:
Zimmermann, F.A., et al. Br. J. Cancer 100(9):1434-1437(2009)
Background Reference 3:
Saada, A., et al. Am. J. Hum. Genet. 82(1):32-38(2008)
Background Reference 4:
Tozlu-Kara, S., et al. J. Mol. Endocrinol. 39(4):305-318(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-779

List Size:
400 ul

List Price:

$495.00

Datasheet
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