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Western blot analysis of extracts of various cell lines, using NDUFB2 antibody (19-236) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of L929 cells using NDUFB2 Polyclonal Antibody (19-236) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NDUFB2 antibody (19-236) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

NDUFB2 Antibody

CATALOG NUMBER: 19-236

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 34-105 of human NDUFB2 (NP_004537.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
NCI-H460
Positive Control 2:
HeLa
Positive Control 3:
293T
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 12kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NDUFB2
Additional Names:
AGGG, CI-AGGG, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa, NADH-ubiquinone oxidoreductase AGGG subunit, complex I AGGG subunit, complex I-AGGG
Ncbi Gene Id Number:
4708
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-236

List Size:
100 uL

List Price:

$436.00

Datasheet
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