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Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of Hela cells stained with NDUFB4 Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Flow cytometric analysis of Hela cells using NDUFB4 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from HepG2, PC-3, Ramos, U-251 MG cell line and human heart tissue lusyte(from left to right), using NDUFB4 Antibody at 1:1000 at each lane.
Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

NDUFB4 Antibody

CATALOG NUMBER: 61-061

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NDUFB4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human NDUFB4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For IF starting dilution is: 1:25
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
15 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDUFB4
Additional Names:
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, CI-B15, NADH-ubiquinone oxidoreductase B15 subunit, NDUFB4
Protein Accession Number:
O95168
Ncbi Gene Id Number:
4710
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Background Reference 1:
Loeffen J.L.C.M.,et al.Biochem. Biophys. Res. Commun. 253:415-422(1998).
Background Reference 2:
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Background Reference 3:
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Background Reference 4:
Murray J.,et al.J. Biol. Chem. 278:13619-13622(2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-061

List Size:
400 ul

List Price:

$469.00

Out of stock

Datasheet
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