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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

NDUFB5 Antibody

CATALOG NUMBER: 30-056

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NDUFB5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NDUFB5 antibody can be used for detection of NDUFB5 by ELISA at 1:62500. NDUFB5 antibody can be used for detection of NDUFB5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
17 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NDUFB5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NDUFB5
Additional Names:
NDUFB5, CI-SGDH, DKFZp686N02262, FLJ30597, MGC111204, MGC12314, SGDH, CISGDH
Protein Accession Number:
NP_002483
Protein Gi Number:
4505363
Ncbi Gene Id Number:
4711
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NDUFB5 is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Background Reference 1:
Ton, C., (1997) Biochem. Biophys. Res. Commun. 241 (2), 589-594.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-056

List Size:
100 ul

List Price:

$519.00

Datasheet
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