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Western blot analysis of extracts of various cell lines, using NDUFB7 antibody (15-154) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of L929 cells using NDUFB7 Polyclonal Antibody (15-154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NDUFB7 antibody (15-154) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

NDUFB7 Antibody

CATALOG NUMBER: 15-154

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human NDUFB7 (NP_004137.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
A-431
Positive Control 2:
HL-60
Positive Control 3:
MCF7
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse heart
Predicted Molecular Weight:
Observed: 16kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NDUFB7
Additional Names:
B18, CI-B18, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa, NADH-ubiquinone oxidoreductase B18 subunit, cell adhesion protein SQM1, complex I B18 subunit, complex I-B18
Ncbi Gene Id Number:
4713
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-154

List Size:
100 uL

List Price:

$436.00

Datasheet
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