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42-625 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-625 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NDUFS2 Antibody

CATALOG NUMBER: 42-625

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-SRGVRQWQPDVE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 48kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 51.9kDa according to NP_001159631.1). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_004541.1; NP_001159631.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 3:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NDUFS2
Additional Names:
NDUFS2, NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase), NADH dehydrogenase (ubiquinone) Fe-S protein 2, NADH-ubiquinone oxidoreductase NDUFS2 subunit, complex 1, mitochondrial respiratory chain, 49-KD subunit
Protein Accession Number:
NP_004541.1,NP_001159631.1
Protein Gi Number:
4758786
Ncbi Gene Id Number:
4720

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-625

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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