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Western blot analysis of extracts of various cell lines, using NDUFS5 antibody (14-561) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using NDUFS5 antibody (14-561) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

NDUFS5 Antibody

CATALOG NUMBER: 14-561

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human NDUFS5 (NP_004543.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:1000 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
LO2
Positive Control 2:
293T
Positive Control 3:
HL-60
Positive Control 4:
HeLa
Positive Control 5:
SW620
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 13kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NDUFS5
Additional Names:
CI-15k, CI15K, NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, CI-15 kDa, NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase), NADH:ubiquinone oxidoreductase 15 kDa IP subunit, complex I-15 kDa
Ncbi Gene Id Number:
4725
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-561

List Size:
50 uL

List Price:

$285.00

Out of stock

Datasheet
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