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Western blot analysis in Hela cell line lysates (35ug/lane).
NDUFS8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela cell line lysates (35ug/lane).

NDUFS8 Antibody

CATALOG NUMBER: 56-500

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, C. Elegans, Monkey, Mouse
Immunogen:
This NDUFS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the Central region of human NDUFS8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDUFS8
Additional Names:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, Complex I-23kD, CI-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, NDUFS8
Protein Accession Number:
O00217
Ncbi Gene Id Number:
4728
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)Bourges, I., et al. Biochem. J. 383 (PT 3), 491-499 (2004)Procaccio, V., et al. Neurology 62(10):1899-1901(2004) Ugalde, C., et al. Hum. Mol. Genet. 13(6):659-667(2004) Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-500

List Size:
400 ul

List Price:

$495.00

Datasheet
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