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Western blot analysis of extracts of HepG2 cells, using NDUFV3 antibody (19-238).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HepG2 cells, using NDUFV3 antibody (19-238).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

NDUFV3 Antibody

CATALOG NUMBER: 19-238

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human NDUFV3 (NP_001001503.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 10kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NDUFV3
Additional Names:
CI-10k, CI-9KD, NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial, NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa, NADH-ubiquinone oxidoreductase 9 kD subunit, NADH-ubiquinone oxidoreductase 9 kDa subunit, NADH-ubiquinone oxidoreductase flavoprotein 3, 10kD, complex I 10kDa subunit, complex I, mitochondrial respiratory chain, 10-kD subunit, complex I-9kD, mitochondrial NADH oxidoreductase-like protein, renal carcinoma antigen NY-REN-4
Ncbi Gene Id Number:
4731
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one of at least forty-one subunits that make up the NADH-ubiquinone oxidoreductase complex. This complex is part of the mitochondrial respiratory chain and serves to catalyze the rotenone-sensitive oxidation of NADH and the reduction of ubiquinone. The encoded protein is one of three proteins found in the flavoprotein fraction of the complex. The specific function of the encoded protein is unknown. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-238

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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