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NEDD8 Recombinant Protein

CATALOG NUMBER: 92-177

Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Met1-Gly76
Fusion Tag:
N-6 His, SUMO tag
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Predicted Molecular Weight:
20.9 kD
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM PB150mM NaCl5% Trehalose,pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Storage Conditions:
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at -20°C for 3 months.
Ncbi Official Symbol:
NEDD8
Additional Names:
Neural precursor cell expressed developmentally down-regulated protein 8, NEDD8, Neddylin, Ubiquitin-like protein Nedd8
Protein Accession Number:
Q15843
Ncbi Gene Id Number:
4738
Background:
Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

92-177

List Size:
0.05 mg

List Price:

$275.00

Datasheet
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