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45-940 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-940 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NEDD9 Antibody

CATALOG NUMBER: 45-940

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: NKPQNKCDDLDR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in Human Kidney, Lung and Placenta lysates (calculated MW of 92.9kDa according to NP_006394.1). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_006394.1) only.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NEDD9
Additional Names:
NEDD9, neural precursor cell expressed, developmentally down-regulated 9, CAS-L, CASL, HEF1, dJ49G10.2, dJ761I2.1, Crk-associated substrate related, cas-like docking dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)), dJ761I2.1 (enhancer of filamentation (HEF1)), enhancer of filamentation 1
Protein Accession Number:
NP_006394.1,NP_892011.2
Protein Gi Number:
5453680
Ncbi Gene Id Number:
4739
Background Reference 1:
Kim M, Gans JD, Nogueira C, Wang A, Paik JH, Feng B, Brennan C, Hahn WC, Cordon-Cardo C, Wagner SN, Flotte TJ, Duncan LM, Granter SR, Chin L. Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene. Cell. 2006 Jun 30;125(7):1269-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-940

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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