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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
NEDD9 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

NEDD9 Antibody

CATALOG NUMBER: 57-842

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NEDD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-363 amino acids from the Central region of human NEDD9.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEDD9
Additional Names:
Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, CAS-L, CasL, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, NEDD-9, Renal carcinoma antigen NY-REN-12, p105, Enhancer of filamentation 1 p55, NEDD9, CASL, CASS2
Protein Accession Number:
Q14511
Protein Gi Number:
8134360
Ncbi Gene Id Number:
4739
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B-and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
Background Reference 1:
Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010)
Background Reference 2:
Lucas, J.T. Jr., et al. Oncogene 29(31):4449-4459(2010)
Background Reference 3:
Tedde, A., et al. Neurosci. Lett. 477(3):121-123(2010)
Background Reference 4:
Malleter, M., et al. Int. J. Mol. Med. 25(6):897-903(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-842

List Size:
400 ul

List Price:

$495.00

Datasheet
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