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Western blot analysis of extracts of mouse brain, using NEFH antibody (23-587) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (23-587) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (23-587) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (23-587) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of mouse brain using NEFH antibody (23-587) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using NEFH antibody (23-587) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse brain, using NEFH antibody (23-587) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

NEFH Antibody

CATALOG NUMBER: 23-587

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse brain
Predicted Molecular Weight:
Observed: 240kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NEFH
Additional Names:
NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
Ncbi Gene Id Number:
4744
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-587

List Size:
100 uL

List Price:

$436.00

Datasheet
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