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45-942 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-942 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NEIL1 Antibody

CATALOG NUMBER: 45-942

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DIPSLEPEGTSAS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10ug/ml.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NEIL1
Additional Names:
NEIL1, NEH1, nei endonuclease VIII-like 1 (E. coli), NEI1, FLJ22402, endonuclease VIII, FPG1, hFPG1, nei endonuclease VIII-like 1
Protein Accession Number:
NP_078884.2
Protein Gi Number:
13375817
Ncbi Gene Id Number:
79661
Background Reference 1:
Hazra TK, Izumi T, Boldogh I, Imhoff B, Kow YW, Jaruga P, Dizdaroglu M, Mitra S. Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA. Proc Natl Acad Sci USA. 2002 Mar 19;99(6):3523-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-942

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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