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Western blot analysis of NEIL1 Antibody in A2058 cell line lysates (35ug/lane)
Western blot analysis of NEIL1 Antibody in A2058 cell line lysates (35ug/lane)

NEIL1 Antibody

CATALOG NUMBER: 64-067

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NEIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the Central region of human NEIL1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEIL1
Additional Names:
Endonuclease 8-like 1, 322-, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, NEH1, Nei-like protein 1, NEIL1
Protein Accession Number:
Q96FI4
Protein Gi Number:
56404654
Ncbi Gene Id Number:
79661
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).
Background Reference 1:
Zhao, X., et al. Biochemistry 49(8):1658-1666(2010)
Background Reference 2:
Forsbring, M., et al. Carcinogenesis 30(7):1147-1154(2009)
Background Reference 3:
Couve, S., et al. J. Biol. Chem. 284(18):11963-11970(2009)
Background Reference 4:
Muftuoglu, M., et al. J. Biol. Chem. 284(14):9270-9279(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-067

List Size:
400 ul

List Price:

$495.00

Datasheet
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