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Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

NEIL3 Antibody

CATALOG NUMBER: 60-519

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NEIL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-320 amino acids from the Central region of human NEIL3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEIL3
Additional Names:
Endonuclease 8-like 3, 322-, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3, NEIL3
Protein Accession Number:
Q8TAT5
Protein Gi Number:
302393810
Ncbi Gene Id Number:
55247
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NEIL3 belongs to a class of DNA glycosylases homologous tothe bacterial Fpg/Nei family. These glycosylases initiate the firststep in base excision repair by cleaving bases damaged by reactiveoxygen species and introducing a DNA strand break via theassociated lyase reaction (Bandaru et al., 2002 [PubMed12509226]).
Background Reference 1:
Krokeide, S.Z., et al. Protein Expr. Purif. 65(2):160-164(2009)
Background Reference 2:
Takao, M., et al. Genes Cells 14(2):261-270(2009)
Background Reference 3:
Dallosso, A.R., et al. Gut 57(9):1252-1255(2008)
Background Reference 4:
Bethke, L., et al. J. Natl. Cancer Inst. 100(4):270-276(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-519

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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