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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of NEK3 using rabbit polyclonal NEK3 Antibody (K346) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NEK3 gene.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane.

NEK3 Antibody

CATALOG NUMBER: 63-381

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NEK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the Central region of human NEK3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEK3
Additional Names:
Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, NEK3
Protein Accession Number:
P51956
Protein Gi Number:
20178297
Ncbi Gene Id Number:
4752
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. NEK3 is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm.
Background Reference 1:
Schultz, S.J., et al., Cell Growth Differ. 5(6):625-635 (1994).
Background Reference 2:
Schultz, S.J., et al., Cell Growth Differ. 4(10):821-830 (1993).
Background Reference 3:
Kimura, M., et al., Cytogenet. Cell Genet. 95 (3-4), 177-182 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-381

List Size:
400 ul

List Price:

$495.00

Datasheet
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