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Antibody is used in Western blot to detect NEK8 in A2058 cell lysate.
Antibody is used in Western blot to detect NEK8 in A2058 cell lysate.

NEK8 Antibody

CATALOG NUMBER: 62-657

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NEK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573-604 amino acids from the C-terminal region of human NEK8.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEK8
Additional Names:
Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nima-related protein kinase 12a, NEK8, JCK, NEK12A
Protein Accession Number:
Q86SG6
Protein Gi Number:
34098463
Ncbi Gene Id Number:
284086
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NEK8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystickidney disease in humans.
Background Reference 1:
Valkova,N. et al. Mol. Cell Proteomics(7), 1009-1018 (2005)
Background Reference 2:
Bowers,A.J. et al. Gene 328, 135-142 (2004)
Background Reference 3:
Holland,P.M. et al. J. Biol. Chem. 277 (18), 16229-16240 (2002)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-657

List Size:
400 ul

List Price:

$495.00

Datasheet
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