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43-562 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-562 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NELL2 Antibody

CATALOG NUMBER: 43-562

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DPCQADTIRNDITKT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Brain (cortex and hippocampus) lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 94.7kDa for the mature form (aa22-866) according to NP_001138579.1 and 88.8kDa (aa22-816) according to NP_001138580.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_001138579.1; NP_006150.1; NP_001138581.1; NP_001138582.1). Reported variants represent identical protein: NP_001138580.1, NP_006150.1.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NELL2
Additional Names:
NELL2, NEL-like 2 (chicken), NRP2, NEL-like protein 2, NEL-related protein 2, neural epidermal growth factor-like 2, protein kinase C-binding protein NELL2
Protein Accession Number:
NP_001138579.1,NP_006150.1,NP_001138581.1,NP_001138582.1
Protein Gi Number:
5453766
Ncbi Gene Id Number:
4753
Background Reference 1:
Kahle JJ, Gulbahce N, Shaw CA, Lim J, Hill DE, Barabási AL, Zoghbi HY.Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-562

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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