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Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 μg/mL.
Immunofluorescence of NENF in rat kidney tissue with NENF antibody at 20 μg/mL.
Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NENF Antibody

CATALOG NUMBER: 4753

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF.
The immunogen is located within the last 50 amino acids of NENF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Purification:
NENF Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NENF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NENF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NENF
Additional Names:
NENF Antibody: CIR2, SPUF, SCIRP10, CIR2, Neudesin, Cell immortalization-related protein 2, SPUF protein
Protein Accession Number:
NP_037481
Protein Gi Number:
7019545
Ncbi Gene Id Number:
29937
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NENF Antibody: NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
Background Reference 1:
Kimura I, Yoshioka M, Konishi M, et al. Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity. J. Neurosci. Res.2005; 79:287-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4753

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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