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Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 μg/mL.
Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 μg/mL.
Trial Size

Nephrin Antibody

CATALOG NUMBER: 5615

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%), Rat: (94%)
Immunogen:
Nephrin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Nephrin.
The immunogen is located within amino acids 870 - 920 of Nephrin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P
Application Note:
Nephrin antibody can be used for detection of Nephrin by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
Nephrin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Nephrin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NPHS1
Additional Names:
Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor
Protein Accession Number:
NP_004637
Protein Gi Number:
4758822
Ncbi Gene Id Number:
4868
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
Background Reference 1:
Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein - Nephrin - is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
Background Reference 2:
Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5
Background Reference 3:
Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5615

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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