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45-947 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
45-947 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

NET1 Antibody

CATALOG NUMBER: 45-947

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-DKALSGGKRKETLV
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:In paraffin embedded Human Tonsil shows nuclear staining of many cells in the interfollicular area of the tonsil. Macrophages in germinal centres of secondary follicles are also positive. Recommended concentration, 1-2ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Positive Control 1:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NET1
Additional Names:
NET1, ARHGEF8, NET1A, neuroepithelial cell transforming gene 1, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelial cell transforming 1, RP11-116G8.1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator
Protein Accession Number:
NP_001040625.1,NP_005854.2
Protein Gi Number:
114145479
Ncbi Gene Id Number:
10276
Background Reference 1:
Chan AM, Takai S, Yamada K, Miki T. Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. Oncogene. 1996 Mar 21;12(6):1259-66.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-947

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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