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45-948 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-948 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NET1 Antibody

CATALOG NUMBER: 45-948

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DKLEYLDEKQRDPR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NET1
Additional Names:
NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator
Protein Accession Number:
NP_005854.2,NP_001040625.1
Protein Gi Number:
19923327
Ncbi Gene Id Number:
10276
Background Reference 1:
Qin H, Carr HS, Wu X, Muallem D, Tran NH, Frost JA. Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1. J Biol Chem. 2005 Mar 4;280(9):7603-13. Epub 2004 Dec 16.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-948

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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