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Western blot analysis of NETO1 in human brain tissue lysate with NETO1 antibody at 0.25 μg/mL.
Immunohistochemistry of NETO1 in rat lung tissue with NETO1 antibody at 2.5 μg/mL.
Immunofluorescence of NETO1 in rat lung tissue with NETO1 antibody at 20 μg/mL.
Western blot analysis of NETO1 in human brain tissue lysate with NETO1 antibody at 0.25 μg/mL.
Trial Size

NETO1 Antibody

CATALOG NUMBER: 6469

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
NETO1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NETO1.
The immunogen is located within amino acids 350 - 400 of NETO1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
NETO1 antibody is predicted to not cross-react with other NETO protein family members.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 60 kDa
Observed: 60 kDa
Purification:
NETO1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NETO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NETO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NETO1
Additional Names:
NETO1 Antibody: BCTL1, BTCL1, Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1
Protein Accession Number:
NP_694821
Protein Gi Number:
20452470
Ncbi Gene Id Number:
81832
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NETO1 Antibody: Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.
Background Reference 1:
Michishita M, Ikeda T, Nakashiba T, et al. A novel gene, Btcl1, encoding CUB and LDLa domains is expressed in restricted areas of mouse brain. Biochem. Biophys. Res. Commun. 2003; 306:680-6.
Background Reference 2:
Stöhr H, Berger C, Fröhlich S, et al. A novel gene encoding a putative transmembrane protein with two extracellular CUB domains and a low-density lipoprotein class A module: isolation of alternatively spliced isoforms in retina and brain. Gene 2002; 286:223-31
Background Reference 3:
Straub C, Hunt DL, Yamasaki M, et al. Distinct functions of kainate receptors in the brain are determined by the auxiliary subunit Neto1. Nat. Neurosci. 2011; 14:866-73.
Background Reference 4:
Ng D, Pitcher GM, Szilard RK, et al. Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning. PLoS Biol. 2009; 7:e41

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6469

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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