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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in HepG2 cell line lysates (35ug/lane).

NEU1 Antibody

CATALOG NUMBER: 60-825

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This NEU1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-214 amino acids from the Central region of human NEU1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEU1
Additional Names:
Sialidase-1, Acetylneuraminyl hydrolase, G9 sialidase, Lysosomal sialidase, N-acetyl-alpha-neuraminidase 1, NEU1, NANH
Protein Accession Number:
Q99519
Protein Gi Number:
17368612
Ncbi Gene Id Number:
4758
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a lysosomal enzymethat cleaves terminal sialic acid residues from substrates such asglycoproteins and glycolipids. In the lysosome, this enzyme is partof a heterotrimeric complex together with beta-galactosidase andcathepsin A (the latter is also referred to as 'protectiveprotein'). Mutations in this gene can lead to sialidosis, alysosomal storage disease that can be type 1 (cherry redspot-myoclonus syndrome or normosomatic type), which is late-onset,or type 2 (the dysmorphic type), which occurs at an earlier agewith increased severity.
Background Reference 1:
Caciotti, A., et al. J. Neurol. 256(11):1911-1915(2009)
Background Reference 2:
Bonten, E.J., et al. J. Biol. Chem. 284(41):28430-28441(2009)
Background Reference 3:
Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009) :
Background Reference 4:
Wang, J., et al. J. Neurochem. 111(2):547-554(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-825

List Size:
400 ul

List Price:

$495.00

Datasheet
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