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Western Blot at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A549 cell line lysates (35ug/lane).
NEU2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of NEU2 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 ug per lane.

NEU2 Antibody

CATALOG NUMBER: 56-269

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NEU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human NEU2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEU2
Additional Names:
Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2
Protein Accession Number:
Q9Y3R4
Protein Gi Number:
229462907
Ncbi Gene Id Number:
4759
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to a family of glycohydrolytic enzymeswhich remove sialic acid residues from glycoproteins andglycolipids. Expression studies in COS7 cells confirmed that thisgene encodes a functional sialidase. Its cytosolic localization wasdemonstrated by cell fractionation experiments. [provided byRefSeq].
Background Reference 1:
Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009)
Background Reference 2:
Li, C.Y., et al. Cell Res. 17(4):357-362(2007)
Background Reference 3:
Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005)
Background Reference 4:
Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-269

List Size:
400 ul

List Price:

$495.00

Datasheet
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