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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with 46-692(0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 46-692(1mg/ml) in Lane B. Detected
46-692 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with 46-692(0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 46-692(1mg/ml) in Lane B. Detected

Neurexin 1 Antibody

CATALOG NUMBER: 46-692

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KEKQPSSAKSSNKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NRXN1
Additional Names:
neurexin 1, DKFZp313P2036, NRXN1, FLJ35941, Hs.22998, KIAA0578, neurexin I
Protein Accession Number:
NP_004792.1,NP_620072.1
Protein Gi Number:
14149613
Ncbi Gene Id Number:
9378
Background Reference 1:
Feng J, Schroer R, Yan J, Song W, Yang C, Bockholt A, Cook EH Jr, Skinner C, Schwartz CE, Sommer SS. High frequency of neurexin 1beta signal peptide structural variants in patients with autism. Neurosci Lett. 2006 Nov 27;409(1):10-3. Epub 2006 Oct 10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-692

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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