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Western blot analysis in Y79 cell line ,mouse cerebellum and rat brain tissue lysates (35ug/lane).
Western blot analysis in Y79 cell line ,mouse cerebellum and rat brain tissue lysates (35ug/lane).

NeuroD1 Antibody

CATALOG NUMBER: 60-933

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
This NeuroD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-348 amino acids from the C-terminal region of human NeuroD1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NEUROD1
Additional Names:
Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3, NEUROD1, BHLHA3, NEUROD
Protein Accession Number:
Q13562
Protein Gi Number:
311033428
Ncbi Gene Id Number:
4760
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.
Background Reference 1:
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Miyachi, T., et al., Brain Res. Mol. Brain Res. 69(2):223-231 (1999). Malecki, M.T., et al., Nat. Genet. 23(3):323-328 (1999). Acharya, H.R., et al., Biochem. Biophys. Res. Commun. 233(2):459-463 (1997). Yokoyama, M., et al., Brain Res. Mol. Brain Res. 42(1):135-139 (1996).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-933

List Size:
400 ul

List Price:

$495.00

Datasheet
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