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Human cerebellum stained with Neurofilament antibody (NF421).
SDS-PAGE Analysis of Purified, BSA-Free Neurofilament Antibody (clone NF421). Confirmation of Integrity and Purity of the Antibody.
Human cerebellum stained with Neurofilament antibody (NF421).

Neurofilament Antibody [NF421]

CATALOG NUMBER: 33-179

Clonality:
Monoclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Mouse
Species Reactivity:
Chicken, Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Chicken, Human, Mouse, Pig, Rat
Immunogen:
Human recombinant Neurofilament protein was used as the immunogen. (1)
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (2)
Prediluted format : incubate for 30 min at RT (3)
The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G purified Neurofilament antibody
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
NEFH
Additional Names:
NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
Ncbi Gene Id Number:
4744
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-179

List Size:
100 ug

List Price:

$626.00

Datasheet
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