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45-958 (0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Neuregulin3 and probed with 45-958 (mock transfection in first lane).
45-958 (0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Neuroligin 3 Antibody

CATALOG NUMBER: 45-958

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RKDKRRQEPLRQPS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 85-90kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 91.6kDa according to NP_061850.2). In transfected HEK293 transiently expressing Neuregulin3 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.05-0.2ug/ml.
Positive Control 1:
Cat. No. XBL-10131 - Human Amygdala Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NLGN3
Additional Names:
neuroligin 3, KIAA1480, ASPGX1, AUTSX1, HNL3, NLGN3
Protein Accession Number:
NP_061850.2
Protein Gi Number:
51593088
Ncbi Gene Id Number:
54413
Background Reference 1:
Tabuchi K, Blundell J, Etherton MR, Hammer RE, Liu X, Powell CM, Sudhof TC. A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science. 2007 Oct 5;318(5847):71-6. Epub 2007 Sep 6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-958

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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