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43-393 (1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-393 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-393 (1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

neuronal pentraxin 1 Antibody

CATALOG NUMBER: 43-393

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ERVKIETALTSLHQR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Brain (Amygdala and Frontal Cortex) lysates (calculated MW of 47.1kDa according to NP_002513.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Additional bands of unknown identity was also consistently observed at 38 and 14kDa. These bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 50kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
neuronal pentraxin 1
Additional Names:
NPTX1, neuronal pentraxin I, NP1, NP-I, neuronal pentraxin-1
Protein Accession Number:
NP_002513.2
Protein Gi Number:
55770878
Ncbi Gene Id Number:
4884
Background Reference 1:
Abad MA, Enguita M, DeGregorio-Rocasolano N, Ferrer I, Trullas R.Neuronal pentraxin 1 contributes to the neuronal damage evoked by amyloid-beta and is overexpressed in dystrophic neurites in Alzheimer's brain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-393

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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