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To detect hNeuroserpin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Neuroserpin (38-296) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.
To detect hNeuroserpin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeuroserpin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hNeuroserpin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeuroserpin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hNeuroserpin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Neuroserpin (38-296) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.

Neuroserpin Antibody

CATALOG NUMBER: 38-203

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNeuroserpin (human Neuroserpin). Human Neuroserpin specific antibody was purified by affinity chromatography employing immobilized hNeuroserpin matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect hNeuroserpin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.
Sandwich
To detect hNeuroserpin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Neuroserpin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.
Western Blot:
To detect hNeuroserpin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeuroserpin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Neuroserpin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
SERPINI1
Additional Names:
PI12, neuroserpin, PI12, Neuroserpin, Peptidase inhibitor 12, PI-12
Protein Accession Number:
Q99574
Protein Gi Number:
3183087
Ncbi Gene Id Number:
5274
User Note:
Centrifuge vial prior to opening.
Background:
Neuroserpin is a member of the serpin superfamily and is predominantly expressed by neurons of the developing and adult brain. This serpin is secreted from axonal growth cones of the CNS and PNS where it inhibits the enzyme tissue plasminogen activator. The expression pattern of neuroserpin and its in vivo inhibitory activity implicate this serpin in regulating axonal growth, reducing seizure activity, controlling damage in cerebral infarction and regulating emotional behaviour and memory. Four different mutations have been described in the human neuroserpin gene that promote neuroserpin abnormal polymerization, giving rise to intraneuronal inclusion bodies and a new form of dementia called Familial Encephalopathy with Neuroserpin Inclusion Bodies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-203

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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