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45-961 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-961 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-961 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Neuroserpin Antibody

CATALOG NUMBER: 45-961

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TMNTSGHDFEEL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Brain lysate (calculated MW of 46.4kDa according to NP_005016.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Specificity:
Reported variants represent identical protein (NP_005016.1; NP_001116224.1).
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SERPINI1
Additional Names:
SERPINI1, neuroserpin, PI12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1, protease inhibitor 12 (neuroserpin), serpin peptidase inhibitor, clade I (neuroserpin), member 1, DKFZp781N13156
Protein Accession Number:
NP_005016.1
Protein Gi Number:
4826904
Ncbi Gene Id Number:
5274
Background Reference 1:
Onda M, Belorgey D, Sharp LK, Lomas DA. Latent Ser49Pro neuroserpin forms polymers in the dementia familial encephalopathy with neuroserpin inclusion bodies. J Biol Chem. 2005 Jan 21;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-961

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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