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Western blot analysis of extracts of various cell lines, using NEXN antibody (14-810) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using NEXN antibody (14-810) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

NEXN Antibody

CATALOG NUMBER: 14-810

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 516-675 of human NEXN (NP_653174.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
SGC-7901
Positive Control 2:
Mouse testis
Positive Control 3:
Rat brain
Positive Control 4:
Rat spleen
Positive Control 5:
Rat testis
Predicted Molecular Weight:
Observed: 81kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NEXN
Additional Names:
NEXN, MGC104234, MGC138865, MGC138866, NELIN, CMH20
Ncbi Gene Id Number:
91624
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a filamentous actin-binding protein that may function in cell adhesion and migration. Mutations in this gene have been associated with dilated cardiomyopathy, also known as CMD1CC. Alternatively spliced transcript variants have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-810

List Size:
100 uL

List Price:

$436.00

Datasheet
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