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Formalin-fixed, paraffin-embedded human cerebellum stained with anti-NF-H antibody (SPM203).
Formalin-fixed, paraffin-embedded human cerebellum stained with anti-NF-H antibody (SPM203).

NF-H Antibody [SPM203]

CATALOG NUMBER: 34-146

Clonality:
Monoclonal
Tested Applications:
Flow, IHC
Host Species:
Mouse
Species Reactivity:
Chicken, Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Chicken, Human, Mouse, Pig, Rat
Immunogen:
The Triton-X 100 insoluble protein fraction from rat brain was used as the immunogen for this anti-NF-H antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the anti-NF-H antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
NEFH
Additional Names:
NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
Ncbi Gene Id Number:
4744
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This mAb reacts with a 200kDa protein, identified as heavy subunit of neurofilaments. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-146

List Size:
100 ug

List Price:

$626.00

Datasheet
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