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Western blot analysis of extracts of various cell lines, using NF-kB p65 antibody (13-175) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using NF-kB p65 antibody (13-175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

NF-kB p65 Antibody

CATALOG NUMBER: 13-175

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide of human NF-kB p65
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:20 - 1:50
IF: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
THP-1
Positive Control 2:
NIH/3T3
Positive Control 3:
BT-474
Positive Control 4:
Mouse lung
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RELA
Additional Names:
RELA, p65, NFKB3
Ncbi Gene Id Number:
5970
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-175

List Size:
100 uL

List Price:

$365.00

Datasheet
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