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42-393 (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-393 (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

NFASC Antibody

CATALOG NUMBER: 42-393

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NFSPNQTKFTVQRT
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in Human Lung lysates (calculated MW of 138kDa according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al, J Biol Chem. 2009 Oct 16;284(42):28533-42. PMID: 19666467). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
Specificity:
This antibody is expected to recognize reported isoforms 1, 2, 3 and 4 (NP_001005388.2;,NP_001153803.1, NP_001153804.1and NP_055905.2 resp) .
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NFASC
Additional Names:
NFASC, neurofascin, DKFZp686P2250, FLJ46866, KIAA0756, NF, NRCAML, OTTHUMP00000065316, OTTHUMP00000065319, OTTHUMP00000065322, OTTHUMP00000065323, OTTHUMP00000065324, neurofascin homolog
Protein Accession Number:
NP_001005388.2,NP_001153803.1,NP_001153804.1,NP_055905.2,
Protein Gi Number:
237858675
Ncbi Gene Id Number:
23114

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-393

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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