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43-582 (0.03ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-582 (0.03ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NFATC3 / NFAT4 Antibody

CATALOG NUMBER: 43-582

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HQELDAHEDDLQIN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 116kDa according to NP_775188.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml.
Specificity:
This antibody is expected to recognize all four reported isoforms (NP_775188.1; NP_004546.1; NP_775186.1; NP_775187.1).
Predicted Molecular Weight:
Approx 150kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NFATC3 / NFAT4
Additional Names:
NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3, NFAT4, NFATX, T cell transcription factor NFAT4, nuclear factor of activated T-cells c3 isoform IE-Xa, nuclear factor of activated T-cells, cytoplasmic 3
Protein Accession Number:
NP_775188.1,NP_004546.1,NP_775186.1,NP_775187.1
Protein Gi Number:
27886561
Ncbi Gene Id Number:
4775
Background Reference 1:
Amasaki Y, Adachi S, Ishida Y, Iwata M, Arai N, Arai K, Miyatake S, A constitutively nuclear form of NFATx shows efficient transactivation activity and induces differentiation of CD4(+)CD8(+) T cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-582

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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