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Western blot analysis of lysates from Jurkat cells, using NFATC3 Rabbit mAb at 1:8000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Confocal imaging of paraffin-embedded Mouse thymus using NFATC3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Rat thymus using NFATC3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.
Western blot analysis of lysates from Jurkat cells, using NFATC3 Rabbit mAb at 1:8000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

NFATC3 Rabbit Recombinant Antibody (82-351)

CATALOG NUMBER: 82-351

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, WB
Application Note:
WB,1:2000 - 1:9000, IF-P,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
116kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NFATC3
Additional Names:
NFAT4; NFATX; NF-AT4c; n339260; NFATC3
Protein Accession Number:
Q12968
Ncbi Gene Id Number:
4775
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-351

List Size:
100 uL

List Price:

$477.00

Datasheet
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